Katie Boulter lost in a deciding set to 28th seed Anastasia Potapova, ending British interest in the singles competitions at the French Open.
Francesca Jones also lost in the second round while Emma Raducanu, Cameron Norrie, Jacob Fearnley and Toby Samuel went out in round one.
Boulter, who was targeting a first appearance in the third round at Roland Garros, came out on top of an untidy opening set which included seven breaks of serve.
However, whereas Potapova found her rhythm on serve, Boulter was broken twice more in each set as the Russian-born Austrian won 5-7 6-4 6-2.
Potapova, who began the clay-court swing outside the top 90 before climbing back up to world number 30, will face defending champion Coco Gauff in round three as she bids to reach the second week of a major for only the second time in her career.
More to follow.